Histology (IHC and ISH)

Histology

The biotechnology department is partnering with Atlantic Fish Tech (FO) and Patogen (NO), to develop histology workflows that Fiskaaling will use for research projects.  In addition to addressing focused research questions, we are planning on routinely sampling and processing tissue as part of the biobanking initiative.  We will focus on building our competence with the following staining methods for Formalin-fixed paraffin embedded tissues (FFPE):

  • H&E (general cellular structure)
  • Periodic acid-Schiff stain (mucus, glycogen, carbohydrate compounds)

Immunohistochemistry

Immunohistochemistry is a valuable tool for identifying where a specific cell type or signaling molecule is located in a tissue.  Determining where a protein is located makes it possible to better understand the protein´s function in the context of physiological processes such as inflammation, nutrient uptake from feed, or growth/development.

Researchers in the biotechnology department have experience in performing immunohistochemistry to identify specific cell types and intra-cellular components.  In particular:

  • Immune cells
  • Nervous system components
  • Intestinal epithelium

In-situ hybridization

In-situ hybridization identifies the location of cell types or signaling molecules based on gene expression using molecular probes generated to recognize an mRNA sequence. This is a valuable tool for validating biomarkers by confirming the identity of the nucleotide sequence within the context of the actual tissue.  In addition, in-situ hybridization is useful when there is no commercial antibody available.

We have established workflows and equipement for performing RNAscope analysis. Probes can be ordered to identify any target in any species as long as the RNA sequence is known.  RNAscope can identify single mRNAs in tissue with virtually no background.  Read more about RNAscope options here: http://rna.acdbio.com/rnascope2.5

Equipment and supplies

The biotechnology department of Fiskaaling and our histology partner, Atlantic Fish Tech, are located in the shared research space at Research Park iNOVA. The laboratory is fully equipped for histological sample processing including a chemical fume hood. Specialized equipement includes:

  • Tissue processing equipment (microtome, tissue processer, paraffin embedder, oven) owned/operated by Atlantic Fish Tech
  • Slide Scanner ( arriving 2021) owned/operated by Atlantic Fish Tech
  • Microscopes owned by Fiskaaling
  • RNAscope hybridization oven owned by Fiskaaling
  • Antigen retrieval equipment owned by Fiskaaling